#Build a construct serial cloner pdf
Much improved Linux version (although still not officially supported)Ī short manual describing Features handling has been updated and added as a pdf file.Adobe Creative Cloud is a suite of software applications and online services enabling graphics creation, editing, sharing and publishing. A bug that made the HelpWindow appear "randomly" Setting-up cohesive ends in the shRNA layout window
Handling of Features when using the Anti-Parallel, Reverse or Complement commands Detection of Att site when right at fragment extremities. Scrolling problems in the Sequence Window when selecting a large fragment. Menu modification : a 'Copy More.' menu has been created to put together alternative Copy functions Menu modification : a new 'Features' menu has been added and Export functions are grouped together in the File menu Better handling of very short (few nucleotides) features. Better parsing of sequence when pasting a NCBI web page in a Sequence window. Better handling of scrolling of the RE list window Copying/Pasting now tries to keep Features Improved parsing of Genbank sequences using the Web Access Window New Keystrokes can be used to select for Particular Enzymes in the Sequence Map (ARROWS, ENTER, ESC) Better handling of scrolling when customizing display in the Sequence map window. List the ORF from the largest to smallest when searching for all ORFs Handling of Feature inheritance when sub-cloning (Build a Construct, Gateway) Resizing the Sequence Window is now possible Warns the user when using the Anti-Parallel, Reverse or Complement commands on only a portion of the sequence An pop-up drawing to illustrate cloning using the Build a Construct window (available by clicking the circled i in the window) New peptide Features added to Serial Cloner collection for automatic scan (e.g. New Preference to show or not the reverse strand by default in the Sequence Map New Preference to set-up the character size to be used in the Sequence Window New Preference to set-up the minimum size of an ORF to be used when searching for all ORFs. New "Copy as Genbank (Full Seq + Features)" menu (Copy More? menu) New "Copy Formatted (Full Seq + Features)" menu to copy the Sequence as formatted in the Sequence Window (Copy More? menu) Create a Feature from a translated sequence Scan for Features directly in the Sequence Map window Display Features in the Sequence Map window. Search for a peptide sequence using the Find window. A companion Software to transform MacVector files to Genbank format compatible with Serial Cloner (from Davide Cittaro) A companion Software to Reset Serial Cloner Preferences in case of problem. Export Sequences in GenBank format (with Features) Import external Features collections (Serial Cloner Format, Generic table format, ApE format) (see the online help accessible from within Serial Cloner to get more details on the different actions) Serial Cloner is still distributed as a freeware although you are welcome to support it. Please, read the short description that is provided with the software.Īnother large list of Additions/Corrections comes with Serial Cloner 2.1 - see below.
Serial Cloner can then import these files. With the MacOSX version also comes a little drop-in application developed by Davide Cittaro that allows to transform MacVector fles to Genbank. Some (fortunately few) users suffered form Preference File corruption, so I simplified for them the correction of this problem. With Serial Cloner 2.1, I also provide an additional little software that you can use to reset Serial Cloner Preferences. I updated the Manual file that describes Features, I strongly encourage you to read it to get familiar with the new possibilities (like definition of peptide Features, new Copy-Paste, new Export format, etc.).Īlso present in version 2.1 are the possibility to directly send BLAST request, to search for a peptide sequence or to expor sequences in a Genbank format. I never do but it was clear that most of you wants to. As was the possibility to resize the Sequence Window. In addition, Features are now shown of the Sequence Map which was an often requested function. A new interface has been developed that also allows to Import/Export Feature Collections. The main enhancement is the possibility to define your own list of Features to be scanned for. But at last, it is there with many improvements and bug fixes. As always, it took longer to release this new version than expected.
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